Curcumin effect on mouse hepatocytes genes MMP-17 & MMP-24
DOI:
https://doi.org/10.5281/zenodo.14216096Keywords:
Hepatocarcinoma, Curcumin, Thioacetamide, MMP17, MMP24Abstract
Introduction: Hepatocellular carcinoma is the most primitive form of liver cancer, which is related to chemo carcinogens such as Thioacetamide (TAA) and molecules of tissue structure changing such as MMPs. Antioxidants such as Curcumin (Cur) can inhibiting these factors. In this research, we will investigate the effect of curcumin on the expression of two MMP-17 and MMP-24 involved in the carcinogenesis of mice after chronic exposure to thioacetamide.
Materials and Methods: In this study, 30 mice were studied in 6 groups of 5, during 4 months. First group: control, second: curcumin, third: TAA, fourth: TAA and curcumin simultaneously. The fifth group: first received TAA for two months and then curcumin for 2 months, finally the sixth group: first curcumin for two months and then TAA. Then mice were then euthanized and their liver tissue were transferred to the laboratory. The results were statistically analyzed by Allel ID (V6) and ANOVA test.
Results: The average has been calculated by SigmaPlot software, showing that MMP17 and MMP24 genes expression were significantly increased by thioacetamide (****p<0001) compared to the control group. Whereas the average level of expression of these genes in other experimental groups which were treated by curcumin simultaneously, or before and after of thioacetamide, showed a significant decrease.
Conclusion: Disruption in arrangement of genes expression due to chemical agents causes tissues to become cancerous. Identifying these factors enables early diagnosis and treatment of the disease. It seems that antioxidants such as curcumin can be used to prevent the harmful effects of toxins. Also, MMPs can be used as prognostic markers in cancer.
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