Metabolic engineering in bacteria using CRISPR–Cas9 genome editing tool
Keywords:
Genome editing, CRISPR, Metabolic engineeringAbstract
Today, it is known that most microorganisms can produce important secondary metabolites for humans, most of which can be used as antibiotics, antifungals, anticancer, etc. Most of these secondary metabolites are produced in pathways where other accessory compounds are also synthesized by some active enzymes in this pathway. Therefore, if it is possible to prevent the synthesis of other side products by using genetic engineering or pathway engineering, the production of the main product can be increased. Among the genome editing tools, the CRISPR Cas9 gene editing system has become one of the most powerful tools with high efficiency in removing competing genes that exist in the pathway of secondary metabolite synthesis.
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