The cytotoxic effects of different polycyclic aromatic hydrocarbons on the antioxidant function of the cultivated hepatocytes from Acanthopagrus arabicus: a comparative study Insert Title here
DOI:
https://doi.org/10.5281/zenodo.14292437کلمات کلیدی:
Naphthalene, Benzo(a)pyrene, Pyrene, total antioxidant capacity, liver enzymes, glutathione, Phenanthreneچکیده
The present study aimed to compare the toxicity of different polycyclic aromatic hydrocarbons, including naphthalene, phenanthrene, pyrene, and benzo(a)pyrene (B(a)P), on viability and the antioxidant function of cultivated hepatocytes from Arabian sea bream (Acanthopagrus arabicus) in vitro. Cultivated hepatocytes from A. arabicus were exposed to different but equal concentrations of four pollutants, including 0 (control), 10-2, 10-1, 1, 10, 102 and 103 μg/ml (in 0.01% DMSO) for 48 h. The results showed that the sensitivity of cultivated hepatocytes to all four pollutants increased in a dose-dependent manner; although, the toxicity of low concentrations of all four pollutants did not show a significant difference with the control cells. Cultured hepatocytes exposed to the highest concentrations of all studied pollutants showed the lowest levels of total antioxidant capacity (TAC) and glutathione content (GSH) and the highest levels of lipid peroxidation (LPO) and alanine transaminase (ALT) activity after 48 h. Naphthalene and B(a)P showed the highest and lowest toxicity, respectively. The level of cytotoxicity, cell viability and the level of antioxidant enzymes as well as liver damage indicator enzymes in the present study were mainly affected by naphthalene followed by phenanthrene, and pyrene and B(a)P had less effect. In conclusion, although all PAHs are able to suppress the antioxidant defense system, PAH compounds with lower molecular weight and fewer benzene rings have a more potential to inhibit antioxidants and increase the level of lipid peroxidation due to their more ability to pass through cell membranes.
مراجع
[1] Livingstone, D.R., Lemaire, P., Matthews, A., Peters, L.D., Porte, C., Fitzpatrick, P.J., Förlin, L., Nasci, C., Fossato, V., Wootton, N. (1995). Assessment of the impact of organic pollutants on goby (Zosterisessor ophiocephalus) and mussel (Mytilus galloprovincialis) from the Venice Lagoon, Italy: biochemical studies. Marine Environmental Research, vol. 39, nos. 1-4, p. 235-240.
[2] Medeiros, P.M., Bicego, M.C., Castelao, R.M., Del Rosso, C., Fillmann, G., Zamboni, A.J. (2005). Natural and anthropogenic hydrocarbon inputs to sediments of Patos Lagoon Estuary. Brazil Environment International, vol. 31, p. 77-87.
[3] Di Giulio, R.T., Hinton, D.E. (2008). The textbook of fishes. CRC Press.
[4] Chomczynski P. (1993). A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques, vol. 15, p. 532 -534.
[5] Selck, H., Handy, R.D., Fernandes, T.F., Klaine, S.J., Petersen, E.J. (2016). Nanomaterials in the aquatic environment: a European Union United States perspective on the status of ecotoxicity testing research priorities, and challenges ahead. Environmental Toxicology and Chemistry, vol. 35, p. 1055-1067.
[6] Segner, H. (1998). Isolation and Primary culture of teleost hepatocytes. Comp. Comparative Biochemistry and Physiology Part A, vol. 120, p. 71-81.
[7] An, R., Li, Y., Niu, X., Yu, H. (2008). Responses of antioxidant enzymes in catfish exposed to lipid crystals from E-waste. International Journal of Environmental Research and Public Health, vol. 5, no. 2, p. 99-103.
[8] Kemnitzer, W., Drewe, J., Jiang, S., Zhang, H., Zhao, J., Crogan- Grundy, C. (2007). Discovery of 4-Aryl-4 H-chromenes as a New Series of Apoptosis Inducers Using a Cell- and Caspase- Based High- Throughput Screening Assay. 3. Structure- Activity Relationships of Fused Rings at the 7. 8- Positions. Journal of Medicinal Chemistry, vol. 50, no. 12, p. 2858-2864.
[9] Momeni, H.R., Soliemani Mehranjani, M., Abnosi, M.H., Nasimi, P. (2010). Evaluation of viability in adult mouse spinal cord slices by MTT. Iranian Journal of Biology, vol. 23, no. 2, p. 267-274.
[10] Schirmer, K., Dixon, D.G., Greenberg, B.M., Bols, N.C. (1998). Ability of 16 priority PAHs to be directly cytotoxic to a cell line from the rainbow trout gill. Toxicol, vol. 127, pp. 129-141.
[11] Castano, A., Becerril, C. (2004). In vitro assessment of DNA damage after short and long-term exposure to benzo[a]pyrene using PARD and the RTG-2 fish cell line. Mutation Research, vol. 552, p. 141-151.
[12] Zhou, B., Liu, Ch., Wang, J., Lam, P.K., Wu, R.S.S. (2006). Primary cultures cells as sensitive in vitro model for assessment of toxic-comparison to hepatocytes and gill epithelia. Aquatic Toxicology, vol. 80, p. 109-118.
[13] Derakhshesh, N., Salamat, N., Movahedinia, A., Hashemitabar, M., Bayati, V. (2019). Exposure of liver cell culture from the orange-spotted grouper, Epinephelus coioides, to benzo[a]pyrene and light results in oxidative damage as measured by antioxidant enzymes. Chemosphere, vol. 226, p. 534-544.
[14] Benzie, F.F., Strain, J.J. (1996). The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay. Analytical Biochemistry, vol. 239, no. 1, p. 70-76.
[15] Paglia, D.E., Valentine, W.N. (1967). Studies on the quantitative and qualitive characterization of erythrocyte glutathione peroxidase. Journal of Laboratory and Clinical Medicine, vol. 70, p. 158-169.
[16] Buege, J.A., Aust, S.D. (1978). Microsomal lipid peroxidation. Methods in Enzymology, vol. 30, p. 302-310.
[17] Reitman, S., Frankel, S. (1957). A colorimertic method for the determination of oxaloacetic acid glutmic pyruvic transminase. In: Roy, S., Bhattacharya, S., 2006. Arsenic induced histopathology and synthesis of stress proteins in liver and kidney of Channa punctatus. Ecotoxicology and Environmental Safety, vol. 65, p. 218-229.
[18] Krogdahl, A., Mckellep, A.B., Baeverfjord, G. (2003). Effects of graded levels of standard soybean metal on intestinal structure, mucosal enzyme activities, and pancreatic response in Atlantic salmon (Salmo salar L.). Aquaculture Nutrition, vol. 9, p. 361–371.
[19] Tuvikene, A. (1995). Responses of fish to polycyclic aromatic hydrocarbons (PAHs). Annales Zoologici Fennici, vol. 32, p. 295–309.
[20] Luís, L., Guilhermino, L. (2012). Short-term toxic effects of naphthalene and pyrene on the common prawn (Palaemon serratus) assessed by a multi-parameter laboratorial approach: mechanisms of toxicity and impairment of individual fitness. Biomarkers, vol. 17, no. 3, p. 275-85.
[21] Yazdani, M. (2018). Comparative toxicity of selected PAHs in rainbow trout hepatocytes: genotoxicity, oxidative stress and cytotoxicity. Drug and Chemical Toxicology. https://doi.org/10.1080/01480545.2018.1497054.
[22] Mitchelmore, C.L., Chipman, J.K. (1998). Detection of DNA strand breaks in brown trout (Salmo trutta) hepatocytes and blood cells using the single cell gel electrophoresis (comet) assay. Aquatic Toxicology, vol. 41, no. 1/2, p. 161–182
[23] Wessel, N., Ménard, D., Pichavant-Rafini, K., Ollivier, H., Le Goff, J., Burgeot, T., Akcha, F. (2010). The toxicity of benzo[a]pyrene on sole (Solea solea) hepatocytes: assessment of genotoxic and enzymatic effects. Polycyclic Aromatic Computation, vol. 30, no. 5, p. 346–354.
[24] Schirmer, K., Chan, A.G., Bols, N.C. (2000). Transitory metabolic disruption and cytotoxicity elicited by benzo[a]pyrene in two cell lines from rainbow trout liver. Journal of Biochemical and Molecular Toxicology, vol. 14, no. 5, p. 262–276
[25] Gad, A.S., Khadrawy, Y.A., El-Nekeety, A.A., Mohamed, S.R., Hassan, N.S., Abel- Wahhab, M.A. (2011). Antioxidant activity and hepatoprotective effects of whey protein and Spirulina in rats. Nutrition, vol. 27, no. 5, p. 582-589.
[26] Pan, L. Q., Ren, J., Liu, J. (2006). Responses of antioxidant systems and LPO level to benzo[a]pyrene and benzo[k]fluranthere in the haemolymph of the scallop Chlamys ferrari. Environmental Pollution, vol. 141, p. 443-451.
[27] Beytut, E., Aksakal, M. (2003). Effects of Dietary Vitamin E and Selenium on Antioxidative Defense Mechanisms in the Liver of Rats Treated with High Doses of Glucocorticoid. Biological Trace Element Research, vol. 91, p. 231-241.
[28] Monari, M., Foschi, J., Motozzo, V., Marin, M. G., Fabbri, M., Rosmini, R., Serrazantti, G.P. (2009). Investigation of EROD, CYP1A immunopositive proteins and SOD in haemocytes of Chamelea gallina and their role in response to B[a]P. Comparative Biochemistry and Physiology Part C, vol. 149, p. 382-392.
[29] Sole, M., Buet, A., Ortiz, L., Maynou, F., Bayona, J.M., Albaiges, J. (2007). Biochemical responses in mussels exposed to the water-accommodated fraction of the "Prestige" fuel oil. Scientia Marina, vol. 71, p. 373-382.
[30] Corredor-Santamaría, W., Calderón-Delgado, I.C., Arbeli, Z., Navas, J.M.., Velasco-Santamaría, Y.M. (2023). Acute effects on hepatic biomarkers in the freshwater native fish Aequidens metae exposed to polycyclic aromatic hydrocarbons. Global Journal of Environmental Science and Management, vol. 9, no. 1, p. 1-14.
[31] Vutukuru, S.S., Prabhath, N.A., Raghavender, M., Yerramilli, A. (2007). Effect of arsenic and chromium on the serum amino-transferaseses activity in Indian major carp, Labeo rohita. International Journal of Environmental Research and Public Health, vol. 4, p. 224-227.
[32] Banaee, M., Sureda, A., Mirvaghefi, A., Ahmadi, K. (2011). Effects of diazon on biochemical parameters of blood in rainbow trout (Oncorhynchus mykiss). Pesticide Biochemistry and Physiology, vol. 99, p. 1-6.
[33] De la Torre, F.R., Salibian, A., Ferrari, L. (2000). Biomarkers assessment in juvenile Cyprinus carpio exposed to waterborne cadmium. Environmental Pollution, vol. 109, p. 277-282.
[34] Kim, S.G., Park, D.K., Jang, S.W., Lee, J.S., Kim, S.S., Chung, M.H. (2008). Effects of Dietary Bezo[a]pyrene on Growth and Hematological Parameters in Juvenile Rockfish, Seabastes schlegeli (Hilgendorf). Bulletin of Environmental Contamination and Toxicology, vol. 81, p. 470-474.
