Microfluidic Porous Silicon Aptasensor Array for Multiplex Detection of Sepsis Biomarkers Using Reflectometric Interference Fourier Transform Spectroscopy (RIFTS)

Sh Mohammadi; F  Rahimi; A.H  Rezayan; A  Abouei Mehrizi; M Sedigh

نویسندگان

Sh Mohammadi Department of Nanobiotechnology and Biomimetics, School of Life Science Engineering, College of Interdisciplinary Science and Technology, University of Tehran, Tehran, Iran نویسنده
F Rahimi Department of Nanobiotechnology and Biomimetics, School of Life Science Engineering, College of Interdisciplinary Science and Technology, University of Tehran, Tehran, Iran نویسنده
A.H Rezayan Department of Nanobiotechnology and Biomimetics, School of Life Science Engineering, College of Interdisciplinary Science and Technology, University of Tehran, Tehran, Iran نویسنده
A Abouei Mehrizi Department of medical technology and tissue engineering, School of Life Science Engineering, College of Interdisciplinary Science and Technology, University of Tehran, Tehran, Iran نویسنده
M Sedigh Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran / Department of Nanomedicine, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran نویسنده

کلمات کلیدی:

Multiplex- Microfluidic, RIFTS- Silicon, Aptasensor, Biomarker- Reflectometric

چکیده

Multiplexing in point-of-care biosensors has revolutionized diagnostics across various fields, including medicine, environmental monitoring, and the food safety, by enabling rapid and simultaneous analysis of multiple analytes. In clinical settings, this technology is particularly crucial for multifactorial, multistage, and potentially fatal conditions like sepsis, a major cause of mortality in intensive care units. Consequently, the development of rapid, accurate, and multiplexed detection methods for sepsis-related biomarkers is crucial for timely diagnosis and informed treatment decisions. Herein, we present a novel label-free aptasensor platform based on porous silicon employs Reflective Interferometric Fourier Transform Spectroscopy (RIFTS), utilizing a 3-channel microfluidic system to multiplex detection of three sepsis biomarkers: C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6). The selectivity of the developed sensing platform was cross-validated versus commonly known interfering non-target proteins without compromising its performance values and in the clinical range of severe sepsis. This innovative approach offers potential for rapid, sensitive, and specific multiplex detection of biomarkers potentially improving patient outcomes through comprehensive and more accurate diagnosis.

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بیوگرافی نویسندگان

Sh Mohammadi، Department of Nanobiotechnology and Biomimetics, School of Life Science Engineering, College of Interdisciplinary Science and Technology, University of Tehran, Tehran, Iran
F Rahimi، Department of Nanobiotechnology and Biomimetics, School of Life Science Engineering, College of Interdisciplinary Science and Technology, University of Tehran, Tehran, Iran
A.H Rezayan، Department of Nanobiotechnology and Biomimetics, School of Life Science Engineering, College of Interdisciplinary Science and Technology, University of Tehran, Tehran, Iran
A Abouei Mehrizi، Department of medical technology and tissue engineering, School of Life Science Engineering, College of Interdisciplinary Science and Technology, University of Tehran, Tehran, Iran
M Sedigh، Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran / Department of Nanomedicine, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran

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