Investigation of Steroidal glycoalkaloid concentrations in different potato cultivars through Liquid chromatography-Tandem Mass Spectrometry

Authors

  • Mashoufi,E Department of Chemistry, Faculty of Science, Imam Khomeini International University, P.O. Box 34148-96818, Qazvin, Iran Author
  • Bagheri M Department of PhysicoChemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran Author
  • Rahmani F Department of Physico Chemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran Author
  • Soleimani, M Department of Physico Chemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran Author
  • Es-haghi,A Department of Physico Chemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran Author

Keywords:

Liquid chromatography-Tandem Mass Spectrometry, Glycoalkaloids, α-Solanine, α-Chaconine, Potato analysis

Abstract

A robust analytical method employing solid-phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) was optimized and validated for the determination of α-solanine and α-chaconine in different potato cultivars. Calibration and validation were conducted using a blank matrix derived from sweet potato powder. The method demonstrated excellent linearity, low detection limits, and high precision and accuracy.

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Author Biographies

  • Mashoufi,E , Department of Chemistry, Faculty of Science, Imam Khomeini International University, P.O. Box 34148-96818, Qazvin, Iran

       

  • Bagheri M, Department of PhysicoChemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran

      

  • Rahmani F, Department of Physico Chemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran

      

  • Soleimani, M, Department of Physico Chemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran

      

  • Es-haghi,A, Department of Physico Chemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran

      

References

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[2] A. M. Kumlay, C. Kaya, M. Olgun, and E. Yildirim, “Molecular genetic techniques assisting to conventional breeding methods in potato improvement,” Acta Hortic., vol. 579, pp. 127–131, 2002, doi: 10.17660/ACTAHORTIC.2002.579.18.

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[4] M. Friedman, K. R. Lee, H. J. Kim, I. S. Lee, and N. Kozukue, “Anticarcinogenic effects of glycoalkaloids from potatoes against human cervical, liver, lymphoma, and stomach cancer cells,” J. Agric. Food Chem., vol. 53, no. 15, pp. 6162–6169, Jul. 2005, doi: 10.1021/JF050620P.

[5] N. Nahar, “Regulation of sterol and glycoalkaloid biosynthesis in potato (Solanum tuberosum L.)-Identification of key genes and enzymatic steps,” 2011.

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[7] G. Bianco, P. Schmitt-Kopplin, G. De Benedetto, A. Kettrup, and T. R. I. Cataldi, “Determination of glycoalkaloids and relative aglycones by nonaqueous capillary electrophoresis coupled with electrospray ionization-ion trap mass spectrometry.,” Electrophoresis, vol. 23, no. 17, pp. 2904–2912, Sep. 2002, doi: 10.1002/1522-2683(200209)23:17<2904::aid-elps2904>3.0.co;2-1.

[8] D. S. McGehee, M. D. Krasowski, D. L. Fung, B. Wilson, G. A. Gronert, and J. Moss, “Cholinesterase inhibition by potato glycoalkaloids slows mivacurium metabolism,” Anesthesiology, vol. 93, no. 2, pp. 510–519, 2000, doi: 10.1097/00000542-200008000-00031.

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Published

2025-01-20

How to Cite

Investigation of Steroidal glycoalkaloid concentrations in different potato cultivars through Liquid chromatography-Tandem Mass Spectrometry. (2025). Development Engineering Conferences Center Articles Database, 2(6). https://pubs.bcnf.ir/index.php/Articles/article/view/328

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