Investigation of Steroidal glycoalkaloid concentrations in different potato cultivars through Liquid chromatography-Tandem Mass Spectrometry

E Mashoufi; M Bagheri; F Rahmani; M Soleimani; A Es-haghi

نویسندگان

E Mashoufi Department of Chemistry, Faculty of Science, Imam Khomeini International University, P.O. Box 34148-96818, Qazvin, Iran نویسنده
M Bagheri Department of PhysicoChemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran نویسنده
F Rahmani Department of Physico Chemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran نویسنده
M Soleimani Department of Physico Chemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran نویسنده
A Es-haghi Department of Physico Chemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran نویسنده

کلمات کلیدی:

Liquid chromatography-Tandem Mass Spectrometry, Glycoalkaloids, α-Solanine, α-Chaconine, Potato analysis

چکیده

A robust analytical method employing solid-phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) was optimized and validated for the determination of α-solanine and α-chaconine in different potato cultivars. Calibration and validation were conducted using a blank matrix derived from sweet potato powder. The method demonstrated excellent linearity, low detection limits, and high precision and accuracy.

دانلودها

دسترسی به دانلود اطلاعات مقدور نیست.

بیوگرافی نویسندگان

E Mashoufi ، Department of Chemistry, Faculty of Science, Imam Khomeini International University, P.O. Box 34148-96818, Qazvin, Iran
M Bagheri، Department of PhysicoChemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran
F Rahmani، Department of Physico Chemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran
M Soleimani، Department of Physico Chemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran
A Es-haghi، Department of Physico Chemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran

مراجع

[1] D. G. Omayio, G. O. Abong, and M. W. Okoth, “A review of occurrence of glycoalkaloids in potato and potato products,” Curr. Res. Nutr. Food Sci., vol. 4, no. 3, pp. 195–202, 2016, doi: 10.12944/CRNFSJ.4.3.05.

[2] A. M. Kumlay, C. Kaya, M. Olgun, and E. Yildirim, “Molecular genetic techniques assisting to conventional breeding methods in potato improvement,” Acta Hortic., vol. 579, pp. 127–131, 2002, doi: 10.17660/ACTAHORTIC.2002.579.18.

[3] J. Urban, K. Hamouz, J. Lachman, J. Pulkrábek, and K. Pazderů, “Effect of genotype, flesh colour and environment on the glycoalkaloid content in potato tubers from integrated agriculture,” https://pse.agriculturejournals.cz/doi/10.17221/166/2018-PSE.html, vol. 64, no. 4, pp. 186–191, 2018, doi: 10.17221/166/2018-PSE.

[4] M. Friedman, K. R. Lee, H. J. Kim, I. S. Lee, and N. Kozukue, “Anticarcinogenic effects of glycoalkaloids from potatoes against human cervical, liver, lymphoma, and stomach cancer cells,” J. Agric. Food Chem., vol. 53, no. 15, pp. 6162–6169, Jul. 2005, doi: 10.1021/JF050620P.

[5] N. Nahar, “Regulation of sterol and glycoalkaloid biosynthesis in potato (Solanum tuberosum L.)-Identification of key genes and enzymatic steps,” 2011.

[6] N. TEK, “Characterization of Glycoalkaloid Content and Molecular Mapping in Eggplant,” Analysis, no. November, 2006.

[7] G. Bianco, P. Schmitt-Kopplin, G. De Benedetto, A. Kettrup, and T. R. I. Cataldi, “Determination of glycoalkaloids and relative aglycones by nonaqueous capillary electrophoresis coupled with electrospray ionization-ion trap mass spectrometry.,” Electrophoresis, vol. 23, no. 17, pp. 2904–2912, Sep. 2002, doi: 10.1002/1522-2683(200209)23:17<2904::aid-elps2904>3.0.co;2-1.

[8] D. S. McGehee, M. D. Krasowski, D. L. Fung, B. Wilson, G. A. Gronert, and J. Moss, “Cholinesterase inhibition by potato glycoalkaloids slows mivacurium metabolism,” Anesthesiology, vol. 93, no. 2, pp. 510–519, 2000, doi: 10.1097/00000542-200008000-00031.